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The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human keratinocytes.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Keratinocytes0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
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CD47 is an adhesion protein that regulates integrin signaling through G protein activation and serves as a receptor for thrombospondin THBS1 affecting signal transduction cardiovascular homeostasis inflammation apoptosis angiogenesis self-renewal and Immunomodulatory It regulates pulmonary endothelin EDN1 signaling acts as a pressor in blood pressure regulation and is critical for memory formation CD47 Protein Human (HEK293) is the recombinant human-derived CD47 protein expressed by HEK293 with tag free
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Hepatocyte, Plateable cryopreserved; Celsis In Vitro Technologies; From female human; Used for induction and toxicity studies; Convenient, quality and characterization; Size: 5 million cells per vial
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For Research Use Only. Human Normal Peripheral Blood Mononuclear Cells (PBMC) - Cryopreserved, 10 million cells/vial. Mononuclear cells (PBMCs) are purified using density gradient centrifugation techniques.
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